Numerical Aperture and Image ResolutionĮxplore how objective numerical aperture size influences Airy disk properties.Įxplores calibration of various eyepiece reticles using a stage micrometer and demonstrates how the reticle can then be employed to determine linear specimen dimensions.Review the basic steps involved in acquiring a STORM super-resolution image. Stochastic Optical Reconstruction Microscopy (STORM) Imaging.Spatial resolution refers to the number of pixels utilized in construction of the image. The Nikon Photo Contest, has been held by Nikon Corporation since 1969 to provide an opportunity for photographers around the world, is to nurture an. Introduction to Phase Contrast MicroscopyĪ mechanism to translate variations in phase into corresponding changes in amplitude.The ratio of the speed of light in a vacuum to that in the imaging medium of a microscope.ĭiscussion of birefringence, Brewster's angle, and various forms of polarized light. The resolving power of a microscope is the most important feature of the optical system. We’re happy to announce that we’ve updated our article on Single-Molecule Super-Resolution Imaging. This article now contains an in-depth discussion of sample preparation, including near-comprehensive tables listing known fluorescent probes and buffer systems for single molecule localization microscopies. For generations, Nikon cameras have been trusted by photographers and picture takers of every caliber for their enduring performance and outstanding image.
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